Journal: Acta Biochimica et Biophysica Sinica
Article Title: Mitochondrial fission factor promotes cisplatin resistancein hepatocellular carcinoma
doi: 10.3724/abbs.2022007
Figure Lengend Snippet: Knockdown of Mff inhibits cell migration and invasion of cisplatin-resistant Huh-7/DDP cells treated with cisplatin Huh-7/DDP cells were transfected/treated with negative control, shMff, cisplatin, or shMff+cisplatin. (A) Cell migration and invasion were analyzed by transwell assay. (B) Cell migration was analyzed by wound-healing assay. (C–E) The expressions of E-cadherin, N-cadherin, MMP-2, MMP-9, CXCR4, and CXCR7 were analyzed by western blot analysis. β-Actin was used as an internal control. Each experiment was repeated three times. *P<0.05.
Article Snippet: The primary antibodies used in the study are listed as follows: rabbit monoclonal antibody to Bcl-2 (ab32124; Abcam), rabbit monoclonal antibody to Bax (ab32503; Abcam), rabbit polyclonal antibody to cleaved-caspase 3 (ab2302; Abcam), rabbit monoclonal antibody to pro-caspase 3 (ab32499; Abcam), rabbit polyclonal antibody to cleaved-caspase 9 (ab2324; Abcam), rabbit polyclonal antibody to pro-caspase 9 (ab138412; Abcam), rabbit monoclonal antibody to cleaved-PARP (ab32064; Abcam), rabbit polyclonal antibody to PARP (ab74290; Abcam), rabbit monoclonal antibody to cytochrome c (ab133504; Abcam), rabbit monoclonal antibody to Hsp60 (ab190828; Abcam), rabbit polyclonal antibody to β-actin (ab8227; Abcam), rabbit monoclonal antibody to E-cadherin (ab40772; Abcam), rabbit monoclonal antibody to N-cadherin (ab76011; Abcam), rabbit monoclonal antibody to MMP-2 (ab92536; Abcam), rabbit monoclonal antibody to MMP-9 (ab76003; Abcam), rabbit monoclonal antibody to COX IV (ab202554; Abcam), rabbit monoclonal antibody to Mff (ab129075; Abcam), rabbit anti-CXCR4 polyclonal antibody (11073-2-AP; Proteintech, Wuhan, China), rabbit anti-CXCR7 polyclonal antibody (20423-1-AP; Proteintech), and rabbit monoclonal antibody to Phospho-MFF (Ser146) (49281; Cell Signaling Technology, Danvers, USA).
Techniques: Knockdown, Migration, Transfection, Negative Control, Transwell Assay, Wound Healing Assay, Western Blot, Control